thr77 Secrets
thr77 Secrets
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in advertising mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two further splice variants were being reported but in-depth nucleotide sequence facts is not yet out there.
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Blocking peptides are peptides that bind particularly to the concentrate on antibody and block antibody binding. These peptide usually contains the epitope recognized through the antibody.
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This manual outlines further possible optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guide outlines even further possible optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
Antibodies certain to the blocking peptide now not bind towards the epitope around the goal protein. By comparing the staining through the blocked antibody as opposed to the antibody by yourself, you can see which staining is precise.
Concentrate on Info Cyclin E belongs on the remarkably conserved cyclin family, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which add on the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This here protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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